Through layers of specialized neurons called photoreceptors, bipolar cells, and ganglion cells, the retina detects light and transmits visual information to the brain. The retina has millions of neuron connections known as synapses. Microglia, which are immune cells of the nervous system, help maintain synaptic integrity. High-acuity color vision depends on cone photoreceptors, which form specialized synapses that swiftly release neurotransmitters to downstream bipolar neurons upon stimulation by light. Zebrafish are an excellent model for studying retinal synapses due to the structural and cellular similarity of their retinas to human retinas, as both have cone-dominant vision. The Mitchell lab previously used electroretinograms (ERGs) to analyze retinal function in microglia-sufficient and microglia-deficient larval zebrafish. These experiments indicate that microglia deficiency alters retinal function through a disruption in the typical time-of-day based changes exhibited by zebrafish. To address these alterations, I studied synaptic scaffolding proteins in microglia-sufficient and -deficient zebrafish retinal tissue at various times of day. I used confocal microscopy to analyze potential differences in synapse scaffold proteins and cone photoreceptor morphology. My results show that microglia-sufficient zebrafish may have less RIBEYE scaffolding protein in the afternoon when compared to the morning. Microglia-deficient zebrafish showed similar amounts of RIBEYE in both morning and afternoon. Cone outer segments appeared longer in microglia-deficient zebrafish in both morning and afternoon when compared to microglia-sufficient zebrafish, and microglia-deficient zebrafish showed increased levels of cone arrestin staining in outer segments in both morning and afternoon; however, additional analysis needs to be done to confirm these results. Cone outer segment length and arrestin staining will be analyzed further by the Mitchell lab. This work can provide new insights into how microglia regulate synaptic processes between cone photoreceptors and their partner retinal neurons.